Profiling of UGT1A1*6, UGT1A1*60, UGT1A1*93, and UGT1A1*28 Polymorphisms in Indonesian Neonates With Hyperbilirubinemia Using Multiplex PCR Sequencing

Messenger Rna Profiling: Body Identification Using Multiplex Real-time Pcr

Conventional methods of body fluid identification use labor-intensive, technologically diverse techniques that are performed in a series, not parallel, manner and are costly in terms of time and sample. Furthermore, for some frequently encountered body fluids no confirmatory technique exists. There is no definitive test, for example, for the presence of saliva or vaginal secretions. In seeking ...

mRNA Profiling: Body Fluid Identification Using Multiplex Real-Time PCR

Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries

Whole transcriptome RNA-sequencing is a powerful tool, but is costly and yields complex data sets that limit its utility in molecular diagnostic testing. A targeted quantitative RNA-sequencing method that is reproducible and reduces the number of sequencing reads required to measure transcripts over the full range of expression would be better suited to diagnostic testing. Toward this goal, we ...

The Frequency and Characteristics of Hypothyroidism Jaundice in Neonates with Hyperbilirubinemia; A Ten-Year Survey

Background & Aims: Congenital hypothyroidism is a serious disorder that could be treated if detected early. Jaundice is one of the symptoms of these neonates. However, the prevalence, clinical course and severity of jaundice are not yet well defined. We compared the clinical course and severity of jaundice in a case-controlstudy of congenital hypothyroidism and other neonates of unknown etiolog...

Phylogeographic analysis of hemorrhagic fever with renal syndrome patients using multiplex PCR-based next generation sequencing

Emerging and re-emerging infectious diseases caused by RNA viruses pose a critical public health threat. Next generation sequencing (NGS) is a powerful technology to define genomic sequences of the viruses. Of particular interest is the use of whole genome sequencing (WGS) to perform phylogeographic analysis, that allows the detection and tracking of the emergence of viral infections. Hantaviru...